Characterization of Recombinant Plasminogen Activator Production by Primate Endothelial Cells Transduced With Retroviral Vectors
نویسندگان
چکیده
Retroviral vector-mediated expression of plasminogen activators (PAS) from endothelial cells (ECs) has been proposed as a potential therapeutic approach for intravascular thrombosis. To define the potential for gene transfer to increase fibrinolytic activity in a primate system, baboon ECs were transduced with retroviral vectors expressing wild-type and glycosylphorphatidylinositol-anchored urokinase, as well as wild-type and serpin-resistant tissue PA (+PA). Expression of either t-PA or urokinase was increased by one log over baseline levels. There was no specific effect of either t-PA or urokinase overexpression on endogenous t-PA. urokinase, or PA inhibitor 1 (PAI-1) expression. Recombinant urokinase could be anchored to the cell surface at a level eight-
منابع مشابه
Characterization of recombinant plasminogen activator production by primate endothelial cells transduced with retroviral vectors.
Retroviral vector-mediated expression of plasminogen activators (PAs) from endothelial cells (ECs) has been proposed as a potential therapeutic approach for intravascular thrombosis. To define the potential for gene transfer to increase fibrinolytic activity in a primate system, baboon ECs were transduced with retroviral vectors expressing wild-type and glycosylphosphatidylinositol-anchored uro...
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